Journal: Molecular Brain

Article Title: Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research

doi: 10.1186/s13041-014-0063-0

Figure Lengend Snippet: List of different primary and secondary antibodies used to characterize the culture

Article Snippet: Post Synaptic Density Protein of 95 kDa (PSD-95) (WB) , 1:3000 , NeuroMab , 75-028 , HRP-conj. Goat anti Mouse , 1:3000 , Rockland , 610-1319.

Techniques: Marker

Journal: Investigative ophthalmology & visual science

Article Title: Cone-like Morphological, Molecular, and Electrophysiological Features of the Photoreceptors of the Nrl Knockout Mouse

doi: 10.1167/iovs.04-1427

Figure Lengend Snippet: Features of Nrl –/– Photoreceptors Compared with Those of WT Mouse Rods and Cones

Article Snippet: Polyclonal antibodies used for immunoblotting were rabbit anti-mouse cone arrestin and rabbit anti-clone transducin alpha (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques:

Journal: Investigative ophthalmology & visual science

Article Title: Cone-like Morphological, Molecular, and Electrophysiological Features of the Photoreceptors of the Nrl Knockout Mouse

doi: 10.1167/iovs.04-1427

Figure Lengend Snippet: Immunoblot analysis of cone phototransduction cascade molecules in the Nrl−/− retina. (A) Immunoblot of the mouse UV-cone pigment (MUV) of a 4-week-old Nrl−/− mouse: lane 1: 1% of the lysate of two Nrl−/− eyes; lanes 2 to 7: twofold incremented amounts of recombinant MUV; the latter runs at a slightly lower molecular mass because of mutations engineered to allow it to be purified with a commercial antibody.12 (B) Plot of the blot densities in (A): each point (●) corresponds to the density of the MUV immunolabeling in the blot lane immediately above it in (A). The symbol corresponding to lane 1 yields the estimate of the MUV mass loaded from the Nrl−/− eye, 1.6 picomoles (arrow projecting to abscissa). Because 1% of the lysate of two eyes was loaded in lane 1, the MUV per eye is thus estimated to be 80 picomoles. (C) Blots comparing extracts of Nrl−/− and WT mouse eyes for cone arrestin (mCarr) and the α-subunit of cone transducin (Gtα2): 25 μg of protein from eyes of animals of each genotype was loaded into adjacent lanes of the gel and immunolabeled. The blot densities of regions circumscribing the immunolabel were quantified, and the ratio of the densities for the blots of the WT and Nrl−/− lanes were determined. The ratios were 14:1 for the mCarr comparison and 11:1 for the Gtα2 comparison in the blots illustrated. Mean ratios (± SEM) were 12.3 ± 1.1 for mCarr (two blots, 11 comparisons of proteins from five Nrl−/− and two WT mice), and 14.1 ± 2.4 for Gtα2 (three blots, 21 comparisons).

Article Snippet: Polyclonal antibodies used for immunoblotting were rabbit anti-mouse cone arrestin and rabbit anti-clone transducin alpha (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Western Blot, Recombinant, Purification, Immunolabeling, Comparison

Journal: iScience

Article Title: LINC00116 -encoded microprotein mitoregulin regulates fatty acid metabolism at the mitochondrial outer membrane

doi: 10.1016/j.isci.2023.107558

Figure Lengend Snippet:

Article Snippet: HRP-conj Goat Anti-Rabbit IgG (H + L) , Jackson ImmunoResearch , Cat# 111-035-003.

Techniques: Recombinant, Purification, Cell Culture, Saline, Protease Inhibitor, Sterility, Titration, Electrophoresis, Control, Western Blot, Isolation, Mass Spectrometry, Software